Mbnl3^tm1.1Sws
Targeted Allele Detail

Summary

• Symbol: Mbnl3^tm1.1Sws
• Name: muscleblind like splicing factor 3; targeted mutation 1.1, Maurice S Swanson
• MGI ID: MGI:5518844
• Synonyms: Mbnl3^deltaE2
• Gene: Mbnl3 Location: ChrX:50206146-50295409 bp, - strand Genetic Position: ChrX, 28.03 cM
• Alliance: Mbnl3^tm1.1Sws page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199127
• Parent Cell Line: CMTI-2 (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Hypomorph, Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. A neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 2. Cre-mediated recombination remove exon 2 and the neomycin resistance cassette. Western blot analysis confirmed the absence of exon 2-containing isoform(s) but over-expression of isoform ZnF3/4 in the E15 forelimb. (J:199127)

Expression

In Mice Carrying this Mutation:

2 assay results 3 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mbnl3 Mutation: 3 strains or lines available

References

• Original: J:199127 Poulos MG, et al., Progressive impairment of muscle regeneration in muscleblind-like 3 isoform knockout mice. Hum Mol Genet. 2013 Sep 1;22(17):3547-58
• All: 4 reference(s)