Trp53^tm1Thst
Targeted Allele Detail

Summary

• Symbol: Trp53^tm1Thst
• Name: transformation related protein 53; targeted mutation 1, Thorsten Stiewe
• MGI ID: MGI:5518935
• Synonyms: p53^LSL-RR, Trp53^LSL-E177R
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm1Thst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198482
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 5 was replaced with a modified one in which nucleotide substitutions (GA-to-CG) result in the amino acid substitution of glutamic acid with arginine at position 177 (p.E177R), mimicking a mutation found in some human tumors. A loxP site flanked puromycin resistance gene cassette was inserted into intron 1. Authors state that this is a null allele. (J:198482)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Trp53^tm1Thst

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 240 strains or lines available

References

• Original: J:198482 Timofeev O, et al., p53 DNA binding cooperativity is essential for apoptosis and tumor suppression in vivo. Cell Rep. 2013 May 30;3(5):1512-25
• All: 2 reference(s)