Tg(Hoxa5-cre)447BLjea
Transgene Detail

Summary

• Symbol: Tg(Hoxa5-cre)447BLjea
• Name: transgene insertion 447B, Lucie Jeannotte
• MGI ID: MGI:5518947
• Synonyms: Tg(14.5kb Hoxa5-cre)Ljea
• Transgene: Tg(Hoxa5-cre)447BLjea Location: unknown
• Alliance: Tg(Hoxa5-cre)447BLjea page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Hoxa5-cre)447BLjea expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Hoxa5

• Mutation details: A 5 kb genomic fragment of Hoxa5 (containing the temporal (T) control region, the brachial spinal cord (BSC) enhancer, proximal Hoxa5 promoter, both exons and the intron of Hoxa5) drives cre epxression in this transgene. An IRES-EGFPcre cassette was inserted into exon 1 of Hoxa5 downstream of the translation initiation codon. A 2.1 kb fragment containing the mesodermal (MES) enhancer and an additional 7.4-kb of downstream sequences including an enhancer driving lung and gut (L/G) expression were added at the 3' end of the construct to produce the 14.5 Hoxa5/Cre transgene. Eight founder lines were obtained and line 447B was established. This line has 6 copies of the transgene. (J:207917)

Generation of Tg(Hoxa5-cre)447BLjea.

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Hoxa5 (species not specified) Summary of all recombinase alleles driven by Hoxa5. Comparative matrix view of recombinase activities

Expression

In Mice Carrying this Mutation:

15 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:207917 Berube-Simard FA, et al., Hoxa5/Cre transgenic mice: novel tools for regional deletion along the anterior-posterior axis. Genesis. 2014 Feb;52(2):149-56
• All: 5 reference(s)