Ninj1^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ninj1^{tm1b(KOMP)Wtsi}
• Name: ninjurin 1; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5519238
• Gene: Ninj1 Location: Chr13:49341005-49349727 bp, + strand Genetic Position: Chr13, 25.14 cM
• Alliance: Ninj1^{tm1b(KOMP)Wtsi} page
• IMPC: Ninj1 gene page

Mutation origin

• Mutant Cell Line: EPD0095_2_C02
• Germline Transmission: Earliest citation of germline transmission: J:197409
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 49346729 of Chromosome 13 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 2 at position 49347711. The exon 2 is thus flanked by loxP sites. Subsequent cre expression resulted in excision of exon 2 which contains most of the coding region. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:197409)

Tumor Data

List all tumor models in MMHCdb carrying Ninj1^{tm1b(KOMP)Wtsi}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ninj1 Mutation: 17 strains or lines available

References

• Original: J:197409 Cho SJ, et al., Ninjurin1, a target of p53, regulates p53 expression and p53-dependent cell survival, senescence, and radiation-induced mortality. Proc Natl Acad Sci U S A. 2013 Jun 4;110(23):9362-7
• All: 2 reference(s)