Col1a1^{tm1(CAG-Ezh2*)Meln}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(CAG-Ezh2*)Meln}
• Name: collagen, type I, alpha 1; targeted mutation 1, Ari Melnick
• MGI ID: MGI:5519911
• Synonyms: Ezh2^Y641N, Ezh2Y641N
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(CAG-Ezh2*)Meln} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199122
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, No functional change, RMCE-ready)
• Mutation: Insertion
• Col1a1^{tm1(CAG-Ezh2*)Meln} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ezh2 (MGI:107940)

• Mutation details: RMCE on KH2 cells inserted a cassette into the 3'UTR that contains a CAG promoter, a STOP/neomycin resistance cassette and cDNA for Ezh2 modified by nucleotide substitution (TAC to AAC) to contain the amino acid substitution asparagine for tyrosine at position 641 (Y641N). (J:199122)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:199122 Beguelin W, et al., EZH2 is required for germinal center formation and somatic EZH2 mutations promote lymphoid transformation. Cancer Cell. 2013 May 13;23(5):677-92
• All: 3 reference(s)