Sestd1^tm1.1Bnrc
Targeted Allele Detail

Summary

• Symbol: Sestd1^tm1.1Bnrc
• Name: SEC14 and spectrin domains 1; targeted mutation 1.1, Benjamin Cheyette
• MGI ID: MGI:5520962
• Gene: Sestd1 Location: Chr2:77010684-77110936 bp, - strand Genetic Position: Chr2, 45.92 cM
• Alliance: Sestd1^tm1.1Bnrc page

Spectrum of posterior defects in Sestd1^tm1.1Bnrc/Sestd1^tm1.1Bnrc and Dact1^tm1.1Bnrc/Dact1^tm1.1Bnrc mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201925
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A loxP site was inserted upstream of exon 4. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 5. Cre-mediated recombination removed exon 4, exon 5 and the neomycin resistance cassette. Western blot analysis confirmed the absence of protein expression in the brain. (J:201925)

Generation of the Sestd1^tm1.1Bnrc allele

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sestd1 Mutation: 32 strains or lines available

References

• Original: J:201925 Yang X, et al., SEC14 and spectrin domains 1 (Sestd1) and Dapper antagonist of catenin 1 (Dact1) scaffold proteins cooperatively regulate the Van Gogh-like 2 (Vangl2) four-pass transmembrane protein and planar cell polarity (PCP) pathway during embryonic development in mice. J Biol Chem. 2013 Jul 12;288(28):20111-20
• All: 2 reference(s)