Igs2^{tm1(ACTB-EGFP,-tdTomato)Zng}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm1(ACTB-EGFP,-tdTomato)Zng}
• Name: intergenic site 2; targeted mutation 1, Hui Zong
• MGI ID: MGI:5520969
• Synonyms: GT11ML, MADM-ML
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm1(ACTB-EGFP,-tdTomato)Zng} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:202665
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The "multiple lox" MADM-ML-11 targeting vector has a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the "GT MADM-ML" cassette. The "GT MADM-ML" cassette is designed with the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; nucleotide 1-273), a beta-globin intronic sequence (containing an frt-lox5171-lox2272-frt-loxP-Neo-loxP), the C-terminal portion of a red fluorescent protein (tdTomato; nucleotide 4-1544) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. (J:101977, J:202665)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 72 strains or lines available

References

• Original: J:202665 Henner A, et al., MADM-ML, a Mouse Genetic Mosaic System with Increased Clonal Efficiency. PLoS One. 2013;8(10):e77672
• All: 11 reference(s)