Oxt^{tm1.1(cre)Dolsn}
Targeted Allele Detail

Summary

• Symbol: Oxt^{tm1.1(cre)Dolsn}
• Name: oxytocin; targeted mutation 1.1, David P Olson
• MGI ID: MGI:5523143
• Synonyms: Oxytocin-Ires Cre
• Gene: Oxt Location: Chr2:130418093-130418974 bp, + strand Genetic Position: Chr2, 63.24 cM
• Alliance: Oxt^{tm1.1(cre)Dolsn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:192007
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Oxt^{tm1.1(cre)Dolsn} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Oxt (MGI:97453)

• Mutation details: A targeting vector was designed to insert an IRES linked to cre recombinase, followed by a Frt-flanked kanamycin resistance gene downstream of the oxytocin (Oxt) stop codon. The construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to flp expressing mice to delete the kanamycin selection cassette. (J:192007)

Generation of Oxt^{tm1.1(cre)Dolsn}.

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Oxt (mouse) Summary of all recombinase alleles driven by Oxt. Comparative matrix view of recombinase activities Alliance mouse Oxt gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Oxt Mutation: 20 strains or lines available

References

• Original: J:192007 Wu Z, et al., An obligate role of oxytocin neurons in diet induced energy expenditure. PLoS One. 2012;7(9):e45167
• All: 56 reference(s)