Porcn^tm1.1Jrt
Targeted Allele Detail

Summary

• Symbol: Porcn^tm1.1Jrt
• Name: porcupine O-acyltransferase; targeted mutation 1.1, Janet Rossant
• MGI ID: MGI:5523157
• Synonyms: Porcn^lox
• Gene: Porcn Location: ChrX:8060087-8072764 bp, - strand Genetic Position: ChrX, 3.7 cM
• Alliance: Porcn^tm1.1Jrt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198636
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: An FRT flanked neomycin cassette followed by a loxP site was inserted in intron 2 and a second loxP site was inserted just downstream of exon 3. Exon 3 is flanked by loxP sites (1154 bp). The resistance cassette was then excised from properly targeted ES cells using flp recombinase and leaving exon 3 flanked by loxP sites. Remaining FRT site 30 bp 5 prime of the 5 prime loxP site (FRT-flanked NeoR cassette excised in vitro). (J:198636)

Expression

In Mice Carrying this Mutation:	31 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Porcn Mutation: 18 strains or lines available

References

• Original: J:198636 Biechele S, et al., Porcn-dependent Wnt signaling is not required prior to mouse gastrulation. Development. 2013 Jul;140(14):2961-71
• All: 10 reference(s)