Exo1^tm3.1Wed
Targeted Allele Detail

Summary

• Symbol: Exo1^tm3.1Wed
• Name: exonuclease 1; targeted mutation 3.1, Winfried Edelmann
• MGI ID: MGI:5523450
• Synonyms: Exo1^EK
• Gene: Exo1 Location: Chr1:175708334-175738962 bp, + strand Genetic Position: Chr1, 81.9 cM
• Alliance: Exo1^tm3.1Wed page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198719
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Humanized sequence) Mutations: Insertion, Single point mutation Mutation details: Glutamic acid codon 109 (GAG) was changed to lysine (AAG) (p.E109K). This mutation corresponds to one found in patients with hereditary nonpolyposis colorectal cancer (HNPCC or Lynch syndrome). Cre-mediated recombination removed the loxP site flanked neomycin resistance cassette that was inserted into intron 5. Western blot analysis confirmed the presence of protein expression in the testis. The product of this allele lacks exonuclease activity. (J:198719)

Gene targeting strategy for the Exo1^tm3.1Wed allele

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Exo1 Mutation: 46 strains or lines available

References

• Original: J:198719 Schaetzlein S, et al., Mammalian Exo1 encodes both structural and catalytic functions that play distinct roles in essential biological processes. Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):E2470-9
• All: 1 reference(s)