Notch1^tm6.1Rko
Targeted Allele Detail

Summary

• Symbol: Notch1^tm6.1Rko
• Name: notch 1; targeted mutation 6.1, Raphael Kopan
• MGI ID: MGI:5523515
• Synonyms: N1¹², N12
• Gene: Notch1 Location: Chr2:26347914-26393834 bp, - strand Genetic Position: Chr2, 18.91 cM
• Alliance: Notch1^tm6.1Rko page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198632
• Parent Cell Line: B6/BLU (ES Cell)
• Strain of Origin: C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Notch1^tm6.1Rko expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Notch2 (MGI:97364)

• Mutation details: A targeting vector constructed by BAC recombineering methods was used to swap the Notch1 intracellular domain (N1ICD) with the ICD of Notch2 (N2ICD) encoding amino acids 1,705 to 2,473, by homologous recombination in B6-derived ES cells. The 3' UTR of Notch1 was not switched so that mRNA stability could be retained. After establishment of germline transmission of this allele, the frt-flanked neo marker that had been introduced as part of the targeting vector was removed by crossing mice to flippase deleter mice. (J:198632)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Notch1 Mutation: 117 strains or lines available

References

• Original: J:198632 Liu Z, et al., The Extracellular Domain of Notch2 Increases Its Cell-Surface Abundance and Ligand Responsiveness during Kidney Development. Dev Cell. 2013 Jun 24;25(6):585-98
• All: 2 reference(s)