Cers6^tm1.1Kwi
Targeted Allele Detail

Summary

• Symbol: Cers6^tm1.1Kwi
• Name: ceramide synthase 6; targeted mutation 1.1, Klaus Willecke
• MGI ID: MGI:5523623
• Gene: Cers6 Location: Chr2:68691785-68944626 bp, + strand Genetic Position: Chr2, 39.53 cM
• Alliance: Cers6^tm1.1Kwi page

Hind limb clasping in Cers6^tm1.1Kwi/Cers6^tm1.1Kwi mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201327
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutation: Insertion Mutation details: 111 bp of the coding region of the catalytically active exon 7 was replaced with a STOP codon, IRES sequence, lacZ reporter gene and FRT flanked neomycin resistance cassette. Flp-mediated recombination removed the neomycin resistance cassette. Western blot analysis confirmed the absence of protein expression in the cerebellum and forebrain. (J:201327)

Generation of the Cers6^tm1.1Kwi allele

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cers6 Mutation: 42 strains or lines available

References

• Original: J:201327 Ebel P, et al., Inactivation of ceramide synthase 6 in mice results in an altered sphingolipid metabolism and behavioral abnormalities. J Biol Chem. 2013 Jul 19;288(29):21433-47
• All: 3 reference(s)