Mir200b^tm1.1Osb
Targeted Allele Detail

Summary

• Symbol: Mir200b^tm1.1Osb
• Name: microRNA 200b; targeted mutation 1.1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:5523790
• Gene: Mir200b Location: Chr4:156140138-156140207 bp, - strand Genetic Position: Chr4, 87.96 cM
• Alliance: Mir200b^tm1.1Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199159
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The microRNA sequence was replaced with a floxed neomycin resistance cassette. Cre-mediated recombination removed the neomycin resistance cassette. Northern blot expression confirmed the absence of microRNA expression. Expression of Mir200a is normal. (J:199159)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Mir200b Mutation: 4 strains or lines available

Notes

This allele was generated in ES cells containing Mir429^tm1Osb.

References

• Original: J:199159 Hasuwa H, et al., miR-200b and miR-429 function in mouse ovulation and are essential for female fertility. Science. 2013 Jul 5;341(6141):71-3
• All: 2 reference(s)