Tg(Wnk4)#Rpl
Transgene Detail

Summary

• Symbol: Tg(Wnk4)#Rpl
• Name: transgene insertion, Richard P Lifton
• MGI ID: MGI:5525134
• Synonyms: Wnk4^WT
• Transgene: Tg(Wnk4)#Rpl Location: unknown
• Alliance: Tg(Wnk4)#Rpl page

Transgene origin

• Strain of Origin: (C3H x C57BL/6)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Wnk4)#Rpl expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Wnk4 (MGI:1917097)

• Mutation details: This transgene consists of a 36.8-kb SwaI fragment of the BAC clone RP23-551L11 (from 129S6/SvEvTac) that contains the complete Wnk4 transcription unit plus 9 kb of 5' flanking and 11.5 kb of 3' flanking sequence. A synonymous substitution at codon 524, creating a new AvaI cleavage site was introduced. Fluorescence in situ hybridization showed a single site of insertion. Transgene expression was detected in heart, kidney, testis, brain, and lung but not in liver or spleen. RT-PCR showed that total amount of mRNA is increased by about 2-fold. The pound symbol (#) is used when line is not specified and/or lines are pooled. (J:113094)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:113094 Lalioti MD, et al., Wnk4 controls blood pressure and potassium homeostasis via regulation of mass and activity of the distal convoluted tubule. Nat Genet. 2006 Oct;38(10):1124-1132
• All: 2 reference(s)