Pde8b^tm1Dgen
Targeted Allele Detail

Summary

• Symbol: Pde8b^tm1Dgen
• Name: phosphodiesterase 8B; targeted mutation 1, Deltagen
• MGI ID: MGI:5526098
• Gene: Pde8b Location: Chr13:95160962-95386844 bp, - strand Genetic Position: Chr13, 49.22 cM
• Alliance: Pde8b^tm1Dgen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:203335
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Deltagen

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 14 and 15, encoding the catalytic domain, were replaced with a lacZ gene with a nuclear localization sequence, a neomycin resistance gene and stop codons. Western blot analysis confirmed the absence of protein expression in the adrenal glands. (J:203335)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pde8b Mutation: 57 strains or lines available

References

• Original: J:203335 Tsai LC, et al., The high-affinity cAMP-specific phosphodiesterase 8B controls steroidogenesis in the mouse adrenal gland. Mol Pharmacol. 2011 Apr;79(4):639-48
• All: 2 reference(s)