Igs11^tm1Tmm
Targeted Allele Detail

Summary

• Symbol: Igs11^tm1Tmm
• Name: intergenic site 11; targeted mutation 1, Thomas M Magin
• MGI ID: MGI:5527249
• Gene: Igs11 Location: unknown Genetic Position: Chr15, Syntenic
• Alliance: Igs11^tm1Tmm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199528
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The mutagenic insertion and chromosome engineering resource (MICER) clone MHPP322c09 was used to insert a loxP site at the 3' end of the keratin type II cluster, downstream of the type I Keratin 18 (Krt18) gene. This clone contains a 6.9 kb insert spanning 101,876,016-101,882,964 bp on chromosome 15 (genome build unknown) and includes a puromycin resistance gene cassette, a loxP site, an HPRT mini-gene, and the agouti (A) coat color gene. The construct was transfected into AB2.2 ES cells carrying Krt1c^tm1Tmm and double-targeted clones in cis were identified for blastocyst injection. Cre-mediated removal of both loxP sites can be used to delete all of the type II keratins and one keratin type I, Krt18. (J:153804)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs11 Mutation: 0 strains or lines available

References

• Original: J:153804 Vijayaraj P, et al., Keratins regulate protein biosynthesis through localization of GLUT1 and -3 upstream of AMP kinase and Raptor. J Cell Biol. 2009 Oct 19;187(2):175-84
• All: 4 reference(s)