Chst14^tm1.1Msch
Targeted Allele Detail

Summary

• Symbol: Chst14^tm1.1Msch
• Name: carbohydrate sulfotransferase 14; targeted mutation 1.1, Melitta Schachner
• MGI ID: MGI:5528395
• Gene: Chst14 Location: Chr2:118756978-118759066 bp, + strand Genetic Position: Chr2, 59.73 cM, cytoband E5
• Alliance: Chst14^tm1.1Msch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:203583
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of the coding exon. An FRT- and loxP-flanked neomycin resistance cassette with a dsRED gene was inserted downstream of the coding exon. Cre-mediated recombination removed the coding exon and neomycin resistance cassette. RT-PCR and northern blot analysis confirmed the absence of transcript expression. (J:203583)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chst14 Mutation: 21 strains or lines available

References

• Original: J:203583 Akyuz N, et al., Dermatan 4-O-sulfotransferase1 ablation accelerates peripheral nerve regeneration. Exp Neurol. 2013 Sep;247:517-30
• All: 2 reference(s)