Mapkapk2^tm1.1Yaff
Targeted Allele Detail

Summary

• Symbol: Mapkapk2^tm1.1Yaff
• Name: MAP kinase-activated protein kinase 2; targeted mutation 1.1, Michael Yaffe
• MGI ID: MGI:5528531
• Synonyms: MK2^CV
• Gene: Mapkapk2 Location: Chr1:130981437-131025563 bp, - strand Genetic Position: Chr1, 56.89 cM, cytoband E4
• Alliance: Mapkapk2^tm1.1Yaff page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:203686
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a loxP site upstream of exon 2, and a second loxP site (in reverse complement sequence to the first loxP site) downstream of exon 2 as well as a frt:PGK-neo:frt sequence downstream of the second loxP site. Because inward-facing loxP sequences result in cre-mediated inversion rather than excision, exon 2 can reversibly switch from Mapkap2 expression orientation (MK2<+CV>, CV; Cre-versible) to the null orientation (MK2<-CV>) and back as long as Cre recombinase is active in the cell.FLP recombinase mediated recombination removed the neomycin cassette. (J:203686)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mapkapk2 Mutation: 35 strains or lines available

References

• Original: J:203686 Morandell S, et al., A Reversible Gene-Targeting Strategy Identifies Synthetic Lethal Interactions between MK2 and p53 in the DNA Damage Response In Vivo. Cell Rep. 2013 Nov 27;5(4):868-77
• All: 3 reference(s)