Atp1a4^tm1Blac
Targeted Allele Detail

Summary

• Symbol: Atp1a4^tm1Blac
• Name: ATPase, Na+/K+ transporting, alpha 4 polypeptide; targeted mutation 1, Gonzalo Blanco
• MGI ID: MGI:5528797
• Synonyms: Atp1a4^null(GFP)
• Gene: Atp1a4 Location: Chr1:172051080-172085981 bp, - strand Genetic Position: Chr1, 79.54 cM
• Alliance: Atp1a4^tm1Blac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:203477
• Parent Cell Line: EDJ22 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An EGFP with a polyadenylation sequence and a floxed neomycin resistance cassette replaced exons 2 through 7. Western blot analysis confirmed the absence of protein expression in the sperm. (J:203477)

Expression

In Mice Carrying this Mutation:	23 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atp1a4 Mutation: 48 strains or lines available

References

• Original: J:203477 McDermott JP, et al., Green fluorescence protein driven by the Na,K-ATPase alpha4 isoform promoter is expressed only in male germ cells of mouse testis. J Assist Reprod Genet. 2012 Dec;29(12):1313-25
• All: 2 reference(s)