Tg(ACTB-THRB*)DSyc
Transgene Detail

Summary

• Symbol: Tg(ACTB-THRB*)DSyc
• Name: transgene insertion D, Sheue-yann Cheng
• MGI ID: MGI:5529515
• Transgene: Tg(ACTB-THRB*)DSyc Location: unknown
• Alliance: Tg(ACTB-THRB*)DSyc page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inserted expressed sequence)
• Mutation: Insertion
• Tg(ACTB-THRB*)DSyc expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  THRB (7068)

  Thrb	(MGI:98743)

• Mutation details: This transgene consists of the human beta-actin promoter driving the expression of a human thyroid hormone beta1 receptor cDNA isolated from the PV kindred with Generalized Resistance to Thyroid Hormone containing a C-insertion at nucleotide 1627 (codon 443). This insertion results in a frame shift of the carboxy terminal 16 amino acids resulting in a total loss of T3-binding and transcriptional activation behaving as a dominant negative. Three founder lines were established: A,D, and G. Line D is a high expressor line. RT-PCR and immunohistochemistry confirmed expression of RNA and protein. (J:134187)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:134187 Wong R, et al., Transgenic mice bearing a human mutant thyroid hormone beta 1 receptor manifest thyroid function anomalies, weight reduction, and hyperactivity. Mol Med. 1997 May;3(5):303-14
• All: 1 reference(s)