Tg(Gcg-icre)23Cshi
Transgene Detail

Summary

• Symbol: Tg(Gcg-icre)23Cshi
• Name: transgene insertion 23, Chiyo Shiota
• MGI ID: MGI:5529611
• Synonyms: Gcg-Cre
• Transgene: Tg(Gcg-icre)23Cshi Location: unknown
• Alliance: Tg(Gcg-icre)23Cshi page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Gcg-icre)23Cshi expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Gcg

• Mutation details: BAC recombineerting was used to generate a construct with a codon-improved cre recombinase sequence inserted into the translation initiation site in exon 2 of the glucagon (Gcg) gene, followed by n adenylation signal sequence. The large first intron of Gcg was replaced with a 300 bp synthetic intron to reduce the size of the final transgene construct that was injected into pronuclei of C57BL/6 zygotes. Founders were screened by PCR for transgene intregration, with 20 and 23 testing positive for integration. Line 23 was chosen for further analysis. (J:203933)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gcg (species not specified) Summary of all recombinase alleles driven by Gcg. Comparative matrix view of recombinase activities

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:203933 Shiota C, et al., alpha-Cells are dispensable in postnatal morphogenesis and maturation of mouse pancreatic islets. Am J Physiol Endocrinol Metab. 2013 Oct 15;305(8):E1030-40
• All: 4 reference(s)