Timm22^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Timm22^{tm1b(KOMP)Wtsi}
• Name: translocase of inner mitochondrial membrane 22; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5529629
• Synonyms: Timm22^-
• Gene: Timm22 Location: Chr11:76297778-76307118 bp, + strand Genetic Position: Chr11, 45.92 cM, cytoband B4
• Alliance: Timm22^{tm1b(KOMP)Wtsi} page
• IMPC: Timm22 gene page

Timm22^{tm1b(KOMP)Wtsi}/Timm22^{tm1b(KOMP)Wtsi} mice exhibit embryonic lethality, with the expected number of embryos at E7.5 but no embryos at E9.5. Embryos at E7.5 are small and form a rudimentary egg-cylinder but show no primitive streak formation or hallmarks of gastrulation.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: EPD0035_1_C02
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 76300011 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site was inserted downstream of the targeted exon(s) at position 76300952. The critical exon(s) were thus flanked by loxP sites, and subsequent Cre-mediated excision deleted the critical sequence and the neomycin selection cassette yielding a knockout reporter construct. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:148605, J:173534, J:188991)

Expression

In Mice Carrying this Mutation:	52 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Timm22 Mutation: 16 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 9 reference(s)