Hcn4^{tm2.1(cre/ERT2)Sev}
Targeted Allele Detail

Summary

• Symbol: Hcn4^{tm2.1(cre/ERT2)Sev}
• Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4; targeted mutation 2.1, Sylvia Evans
• MGI ID: MGI:5529675
• Synonyms: HCN4CreERT2
• Gene: Hcn4 Location: Chr9:58730695-58770458 bp, + strand Genetic Position: Chr9, 31.8 cM, cytoband C
• Alliance: Hcn4^{tm2.1(cre/ERT2)Sev} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204126
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Hcn4^{tm2.1(cre/ERT2)Sev} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Hcn4 (MGI:1298209)

• Mutation details: A targeting vector was designed to insert a CreERT2 coding sequence and frt-flanked neomycin resistance selection cassette immediately before endogenous start codon. The neo cassette was removed by FLP mediated recombination. (J:204126)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Hcn4 (mouse) Summary of all recombinase alleles driven by Hcn4. Alliance mouse Hcn4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hcn4 Mutation: 67 strains or lines available

References

• Original: J:204126 Liang X, et al., HCN4 dynamically marks the first heart field and conduction system precursors. Circ Res. 2013 Aug 2;113(4):399-407
• All: 8 reference(s)