Hcn4^tm3Sev
Targeted Allele Detail

Summary

• Symbol: Hcn4^tm3Sev
• Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4; targeted mutation 3, Sylvia Evans
• MGI ID: MGI:5529677
• Synonyms: HCN4H2BGFP, HCN4nlacZ
• Gene: Hcn4 Location: Chr9:58730695-58770458 bp, + strand Genetic Position: Chr9, 31.8 cM, cytoband C
• Alliance: Hcn4^tm3Sev page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204126
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Humanized sequence, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a floxed nuclear lacZ followed by H2BGFP fusion protein, and frt-flanked neomycin resistance selection cassette, immediately before the endogenous start codon. The H2BGFP fusion protein consists of the coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein (EGFP) gene. (J:204126)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hcn4 Mutation: 67 strains or lines available

References

• Original: J:204126 Liang X, et al., HCN4 dynamically marks the first heart field and conduction system precursors. Circ Res. 2013 Aug 2;113(4):399-407
• All: 4 reference(s)