Tfap2a^tm1.1Hsv
Targeted Allele Detail

Summary

• Symbol: Tfap2a^tm1.1Hsv
• Name: transcription factor AP-2, alpha; targeted mutation 1.1, Henry M Sucov
• MGI ID: MGI:5529780
• Synonyms: AP-2a^ki
• Gene: Tfap2a Location: Chr13:40868778-40891852 bp, - strand Genetic Position: Chr13, 20.01 cM, cytoband A5-B1
• Alliance: Tfap2a^tm1.1Hsv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73813
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An IRES, lacZ and floxed neomycin resistance cassette were inserted into exon 7 via homologous recombination resulting in disruption of the DNA binding domain. Cre-mediated recombination removed the floxed neo cassette. The mutant transcript encodes the first 6 exons of the gene followed by the IRES-lacZ message. The pattern of lacZ expression in mutant animals is similar to that of the endogenous protein. (J:73813)

Expression

In Mice Carrying this Mutation:	36 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tfap2a Mutation: 39 strains or lines available

Notes

The authors indicate that removal of the neomycin cassette by Cre-mediated recombination does not alter gross morphology or lacZ expression pattern. (J:73813)

References

• Original: J:73813 Brewer S, et al., Requirement for AP-2alpha in cardiac outflow tract morphogenesis. Mech Dev. 2002 Jan;110(1-2):139-49
• All: 12 reference(s)