Klf1^tm1.1Ryan
Targeted Allele Detail

Summary

• Symbol: Klf1^tm1.1Ryan
• Name: Kruppel-like transcription factor 1 (erythroid); targeted mutation 1.1, Thomas Ryan
• MGI ID: MGI:5529812
• Synonyms: EGFP-EKLF
• Gene: Klf1 Location: Chr8:85628611-85631920 bp, + strand Genetic Position: Chr8, 41.3 cM
• Alliance: Klf1^tm1.1Ryan page

Mutation origin

• Mouse Generated: Earliest citation of chimera generation: J:204029
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An EGFP gene, with a three glycine codon (GGA GGT GGA) linker to created a fusion, was inserted into exon 1 between nucleotides 3424 and 3425 (GenBank Accession NO. AF019074.1). A floxed hygromycin and kanamycin resistance cassette was inserted downstream of exon 1. Cre-mediated recombination removed the selection cassette. (J:204029)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klf1 Mutation: 22 strains or lines available

References

• Original: J:204029 Liu S, et al., Erythropoiesis in the absence of adult hemoglobin. Mol Cell Biol. 2013 Jun;33(11):2241-51
• All: 1 reference(s)