Tg(Nes-flpo/ERT2)34CAlj
Transgene Detail

Summary

• Symbol: Tg(Nes-flpo/ERT2)34CAlj
• Name: transgene insertion 34C, Alexandra L Joyner
• MGI ID: MGI:5532191
• Synonyms: Nestin-FlpoER, Tg(Nes-flpo/ERT2)1Alj
• Transgene: Tg(Nes-flpo/ERT2)34CAlj Location: unknown
• Alliance: Tg(Nes-flpo/ERT2)34CAlj page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Nes-flpo/ERT2)34CAlj expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Nes

• Mutation details: The transgene construct contained a Flp sequence optimized for translation in mammalian cells (Flpo) joined to the ERT2 mutated estrogen receptor ligand-binding domain by the linker sequence from the Flpe/ERT2 cassette. The founder line 34C, with transgene insertion on Chromosome 15, were used to create the colony (J:186310)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nes (species not specified) Summary of all recombinase alleles driven by Nes.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:186310 Lao Z, et al., MASTR: a technique for mosaic mutant analysis with spatial and temporal control of recombination using conditional floxed alleles in mice. Cell Rep. 2012 Aug 30;2(2):386-96
• All: 9 reference(s)