Hltf^tm1.2Geno
Targeted Allele Detail

Summary

• Symbol: Hltf^tm1.2Geno
• Name: helicase-like transcription factor; targeted mutation 1.2, Genoway
• MGI ID: MGI:5532313
• Gene: Hltf Location: Chr3:20111975-20172654 bp, + strand Genetic Position: Chr3, 6.15 cM
• Alliance: Hltf^tm1.2Geno page

Widespread vacuolation in the anteriorcommissure, preoptic/ventral palladium, and motor cortex of Hltf^tm1.2Geno/Hltf^tm1.2Geno neonatal brains

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:203735
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A loxP site was inserted upstream of exon 11. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 12. Flp-mediated recombination removed the neomycin resistance cassette. Cre-mediated recombination removed exons 11 and 12. Western blot analysis confirmed the absence of protein expression in the heart, lung, liver, kidney and spleen. (J:203735)

Generation of the Hltf^tm1.2Geno allele

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hltf Mutation: 47 strains or lines available

References

• Original: J:203735 Helmer RA, et al., Role of helicase-like transcription factor (hltf) in the G2/m transition and apoptosis in brain. PLoS One. 2013;8(6):e66799
• All: 6 reference(s)