Hprt1^{tm332(Ple272-icre/ERT2)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm332(Ple272-icre/ERT2)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 332, Elizabeth M Simpson
• MGI ID: MGI:5532374
• Synonyms: CLDN5-creERT2, Ple272-icre/ERT2;mEMS5806
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm332(Ple272-icre/ERT2)Ems} page

Mutation origin

• Mutant Cell Line: mEMS5806
• Germline Transmission: Earliest citation of germline transmission: J:204202
• Parent Cell Line: mEMS4857.02 (ES Cell)
• Strain of Origin: B6N.Cg-A^w-J Hprt1^b-m3
• Project Collection: EUCOMMTOOLS

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Hprt1^{tm332(Ple272-icre/ERT2)Ems} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  CLDN5 (7122)

  Cldn5

  (MGI:1276112)

• Mutation details: The transgene consists of a RP11-16.C10 human bacterial artificial chromosome (BAC) retrofitted to contain a human HPRT complementary sequence (containing exon 1, intron 1, exon 2 and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm; all inserted into the sacB gene in the BAC vector. Next, an iCre/ERT2 fusion gene, an intron, an SV40 early polyA signal and an Frt-flanked kanamycin resistance (Kan) cassette were inserted directly at the ATG of the human CLDN5 locus. Transient transfection with FLP recombinase removed the Kan cassette before the modified BAC was used for targeting as a single copy "knock-in" into the upstream region of the Hprt locus. (J:270567)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CLDN5 (human) Summary of all recombinase alleles driven by CLDN5. Alliance human CLDN5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:204202 The Pleiades Promoter and CanEuCre Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2014
• All: 2 reference(s)