Hprt1^{tm352a(Hspa1a-icre/ERT2)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm352a(Hspa1a-icre/ERT2)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 352a, Elizabeth M Simpson
• MGI ID: MGI:5532520
• Synonyms: Hprt^{tm352a(Hsp68-icre/ERT2)Ems}, Hspa1a-creERT2, Ple291-icre/frt/ERT2/frt;mEMS6051
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm352a(Hspa1a-icre/ERT2)Ems} page

Mutation origin

• Mutant Cell Line: mEMS6051
• Germline Transmission: Earliest citation of germline transmission: J:204202
• Parent Cell Line: mEMS4855.02 (ES Cell)
• Strain of Origin: B6N.Cg-A^w-J Hprt1^b-m3
• Project Collection: EUCOMMTOOLS

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Hprt1^{tm352a(Hspa1a-icre/ERT2)Ems} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Hspa1a (MGI:96244)

• Mutation details: The transgene was designed with an HS4 insulator region, the 878 bp Ple291 minipromoter (878 bp from a 5 prime flanking region from the mouse heat shock protein 1A locus (Hspa1a or hsp68) known to contain five HSE and to be sufficient for proper heat induction of the hsp68 gene), a chimeric intron cassette, the iCre/ERT2 fusion gene (with F3-frt sites flanking the ERT2 sequences), the mut6-WPRE element, an SV40 early polyA signal, an additional HS4 insulator region, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. (J:270567)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Hspa1a (mouse) Summary of all recombinase alleles driven by Hspa1a. Alliance mouse Hspa1a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:204202 The Pleiades Promoter and CanEuCre Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2014
• All: 2 reference(s)