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Dnaaf2tm1.1(KOMP)Vlcg
Targeted Allele Detail
Summary
Symbol: Dnaaf2tm1.1(KOMP)Vlcg
Name: dynein, axonemal assembly factor 2; targeted mutation 1.1, Velocigene
MGI ID: MGI:5532530
Synonyms: Dnaaf2-
Gene: Dnaaf2  Location: Chr12:69235861-69245203 bp, - strand  Genetic Position: Chr12, 28.73 cM, cytoband C3
Alliance: Dnaaf2tm1.1(KOMP)Vlcg page
IMPC: Dnaaf2 gene page
Dnaaf2tm1.1(KOMP)Vlcg/Dnaaf2tm1.1(KOMP)Vlcg embryos are small at E7.5 and stall in development at E8.5. Gastrulation occurs but with left-right patterning defects.

Show the 1 phenotype image(s) involving this allele.

Mutation
origin
Mutant Cell Line:  11515B-H12
Germline Transmission:  Earliest citation of germline transmission: J:188991
Parent Cell Line:  VGB6 (ES Cell)
Strain of Origin:  C57BL/6NTac
Project Collection: KOMP-Regeneron
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: ZEN-Ub1
 
Mutation detailsThe insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 8459bp between positions 70290811-70299269 of Chromosome 12 (Genome Build37), and subsequent Cre-mediated excision deleted the neomycin selection cassette. A portion of genomic sequence including exon 1, intron 1 and exon 2, intron 2 and part of exon 3, is thus deleted and replaced with a lacZ reporter gene. RT-PCR analysis confirmed the absence of mRNA expression in homozygous mutant embryos. (J:136110, J:188991, J:278736)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Mice Carrying this Mutation: 50 assay results
In Structures Affected by this Mutation: 10 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Dnaaf2 Mutation:  29 strains or lines available
References
Original:  J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012;
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/05/2024
MGI 6.24
The Jackson Laboratory