Dnaaf2^{tm1.1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Dnaaf2^{tm1.1(KOMP)Vlcg}
• Name: dynein, axonemal assembly factor 2; targeted mutation 1.1, Velocigene
• MGI ID: MGI:5532530
• Synonyms: Dnaaf2^-
• Gene: Dnaaf2 Location: Chr12:69235861-69245203 bp, - strand Genetic Position: Chr12, 28.73 cM, cytoband C3
• Alliance: Dnaaf2^{tm1.1(KOMP)Vlcg} page
• IMPC: Dnaaf2 gene page

Dnaaf2^{tm1.1(KOMP)Vlcg}/Dnaaf2^{tm1.1(KOMP)Vlcg} embryos are small at E7.5 and stall in development at E8.5. Gastrulation occurs but with left-right patterning defects.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: 11515B-H12
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 8459bp between positions 70290811-70299269 of Chromosome 12 (Genome Build37), and subsequent Cre-mediated excision deleted the neomycin selection cassette. A portion of genomic sequence including exon 1, intron 1 and exon 2, intron 2 and part of exon 3, is thus deleted and replaced with a lacZ reporter gene. RT-PCR analysis confirmed the absence of mRNA expression in homozygous mutant embryos. (J:136110, J:188991, J:278736)

Disease models

Expression

In Mice Carrying this Mutation:	50 assay results
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dnaaf2 Mutation: 29 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 7 reference(s)