Nkx2-2^{tm4.1(cre/EGFP)Suss}
Targeted Allele Detail

Summary

• Symbol: Nkx2-2^{tm4.1(cre/EGFP)Suss}
• Name: NK2 homeobox 2; targeted mutation 4.1, Lori Sussel
• MGI ID: MGI:5538250
• Synonyms: Nkx2.2^Cre, Nkx2.2:Cre-EGFP
• Gene: Nkx2-2 Location: Chr2:147019466-147036163 bp, - strand Genetic Position: Chr2, 72.63 cM, cytoband H
• Alliance: Nkx2-2^{tm4.1(cre/EGFP)Suss} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204532
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase)
• Mutations: Insertion, Intragenic deletion
• Nkx2-2^{tm4.1(cre/EGFP)Suss} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nkx2-2 (MGI:97347)

• Mutation details: A cre/EGFP fusion gene and Frt-flanked hygro selection cassette were inserted using recombineering into an Nkx2-2 basal exchange vector such that cre/EGFP was fused in-frame with the ATG start codon, replacing all but 25 base pairs of the first coding exon. This vector was co-injected with a cre-expression vector into ES cells containing an Nkx2-2 (loxed cassette acceptor) allele. Correctly exchanged ES clones were used to produce chimeras which were bred to B6 mice to verify germline transmission. Animals were bred to ACTB-Flpe mice to excise the hygromycin marker. (J:204532)

Generation of Nkx2-2^{tm4.1(cre/EGFP)Suss}.

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nkx2-2 (mouse) Summary of all recombinase alleles driven by Nkx2-2. Comparative matrix view of recombinase activities Alliance mouse Nkx2-2 gene page

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nkx2-2 Mutation: 14 strains or lines available

References

• Original: J:204532 Balderes DA, et al., Nkx2.2:Cre knock-in mouse line: A novel tool for pancreas- and CNS-specific gene deletion. Genesis. 2013 Dec;51(12):844-51
• All: 10 reference(s)