Ank3^tm2.2Bnt
Targeted Allele Detail

Summary

• Symbol: Ank3^tm2.2Bnt
• Name: ankyrin 3, epithelial; targeted mutation 2.2, Vann Bennett
• MGI ID: MGI:5538266
• Gene: Ank3 Location: Chr10:69234608-69863266 bp, + strand Genetic Position: Chr10, 36.1 cM
• Alliance: Ank3^tm2.2Bnt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198370
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

Allele Type: Targeted (Hypomorph, Modified isoform(s)) Mutations: Insertion, Intragenic deletion Mutation details: A loxP site was inserted upstream of exon 22. An FRT-flanked neomycin resistance cassette with a 3' loxp site was inserted downstream of exon 23. Flp-mediated recombination removed the neomycin resistance cassette. Cre-mediated recombination removed exons 22 and 23. Western blot analysis confirmed the loss of the 190-, 270- and 480-kDa isoforms in the brain and the loss of the 190 kDa isoform but retention of the 150, 120 and 90 kDa isoforms in the kidney. (J:198370)

Schematic of the Ank3^tm2.1Bnt and Ank3^tm2.2Bnt alleles

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ank3 Mutation: 170 strains or lines available

References

• Original: J:198370 Jenkins PM, et al., E-cadherin polarity is determined by a multifunction motif mediating lateral membrane retention through ankyrin-G and apical-lateral transcytosis through clathrin. J Biol Chem. 2013 May 17;288(20):14018-31
• All: 2 reference(s)