Pms2^tm2(cre)Lisk
Targeted Allele Detail

Summary

• Symbol: Pms2^tm2(cre)Lisk
• Name: PMS1 homolog2, mismatch repair system component; targeted mutation 2, Michael Liskay
• MGI ID: MGI:5538273
• Synonyms: Pms2^cre, Pms2^Cre
• Gene: Pms2 Location: Chr5:143846782-143870786 bp, + strand Genetic Position: Chr5, 82.82 cM, cytoband G2
• Alliance: Pms2^tm2(cre)Lisk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204653
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Pms2^tm2(cre)Lisk expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pms2 (MGI:104288)

• Mutation details: The SV40 nuclear localization signal (NLS) within the cre sequence was modified to contain an 12-base pair A-T run which preserved the NLS, but added an extra A inducing a +1 bp shift. An IRES was cloned 5' to the cre ATG signal with a neo cassette cloned 3' to the recombinase sequence. The IRES-cre-neo vector was used for homologous recombination in ES cells, resulting in the IRES-cre inserting into the Pms2 locus replacing exon 2. The extra A in the NLS causes the cre to be out of frame (inactive). Because this Pms2 allele is null (thus deficient in DNA mismatch repair), homozygotes will have an increased frequency of -1 bp resulting in cre reversion and thus in cre expression. (J:204653)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pms2 (mouse) Summary of all recombinase alleles driven by Pms2. Comparative matrix view of recombinase activities Alliance mouse Pms2 gene page

Tumor Data

List all tumor models in MMHCdb carrying Pms2^tm2(cre)Lisk

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pms2 Mutation: 53 strains or lines available

References

• Original: J:204653 Miller AJ, et al., Tractable Cre-lox system for stochastic alteration of genes in mice. Nat Methods. 2008 Mar;5(3):227-9
• All: 5 reference(s)