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Pms2tm2(cre)Lisk
Targeted Allele Detail
Summary
Symbol: Pms2tm2(cre)Lisk
Name: PMS1 homolog2, mismatch repair system component; targeted mutation 2, Michael Liskay
MGI ID: MGI:5538273
Synonyms: Pms2cre, Pms2Cre
Gene: Pms2  Location: Chr5:143846782-143870786 bp, + strand  Genetic Position: Chr5, 82.82 cM, cytoband G2
Alliance: Pms2tm2(cre)Lisk page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:204653
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutations:    Insertion, Intragenic deletion
 
Pms2tm2(cre)Lisk expression driven by 1 gene
 
Mutation detailsThe SV40 nuclear localization signal (NLS) within the cre sequence was modified to contain an 12-base pair A-T run which preserved the NLS, but added an extra A inducing a +1 bp shift. An IRES was cloned 5' to the cre ATG signal with a neo cassette cloned 3' to the recombinase sequence. The IRES-cre-neo vector was used for homologous recombination in ES cells, resulting in the IRES-cre inserting into the Pms2 locus replacing exon 2. The extra A in the NLS causes the cre to be out of frame (inactive). Because this Pms2 allele is null (thus deficient in DNA mismatch repair), homozygotes will have an increased frequency of -1 bp resulting in cre reversion and thus in cre expression. (J:204653)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Pms2 (mouse)
Summary of all recombinase alleles driven by Pms2.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Tumor Data
List all tumor models in MMHCdb carrying Pms2tm2(cre)Lisk
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Pms2 Mutation:  53 strains or lines available
References
Original:  J:204653 Miller AJ, et al., Tractable Cre-lox system for stochastic alteration of genes in mice. Nat Methods. 2008 Mar;5(3):227-9
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory