Tbx3^tm3.2Moon
Targeted Allele Detail

Summary

• Symbol: Tbx3^tm3.2Moon
• Name: T-box 3; targeted mutation 3.2, Anne M Moon
• MGI ID: MGI:5538565
• Synonyms: Tbx3^deltaflox
• Gene: Tbx3 Location: Chr5:119808734-119822789 bp, + strand Genetic Position: Chr5, 60.34 cM
• Alliance: Tbx3^tm3.2Moon page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:179977
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A loxA1 site was inserted upstream of exon 1. An FRT-flanked neomycin resistance cassette with a 5' loxA1 site was inserted in intron 1. Flp-mediated recombination removed the neomycin resistance cassette. Cre-mediated recombination removed exon 1. Immunohistochemistry confirmed the absence of protein expression in the sinoatrial node. (J:179977)

Generation of the Tbx3^tm1.1Moon, Tbx3^tm2Moon, Tbx3^tm2.1Moon, Tbx3^tm3.1Moon, and Tbx3^tm3.2Moon alleles

Expression

In Mice Carrying this Mutation:	243 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbx3 Mutation: 49 strains or lines available

References

• Original: J:179977 Frank DU, et al., Lethal arrhythmias in Tbx3-deficient mice reveal extreme dosage sensitivity of cardiac conduction system function and homeostasis. Proc Natl Acad Sci U S A. 2012 Jan 17;109(3):E154-63
• All: 5 reference(s)