Slirp^tm1.2Pjle
Targeted Allele Detail

Summary

• Symbol: Slirp^tm1.2Pjle
• Name: SRA stem-loop interacting RNA binding protein; targeted mutation 1.2, Peter J Leedman
• MGI ID: MGI:5538716
• Gene: Slirp Location: Chr12:87490666-87496694 bp, + strand Genetic Position: Chr12, 41.67 cM, cytoband E
• Alliance: Slirp^tm1.2Pjle page

Annulus and mitochondrial disruption in Slirp^tm1.2Pjle/Slirp^tm1.2Pjle sperm

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204433
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: Exon 2 was replaced with loxP-flanked cDNA for exons 2 to 4, with splice acceptor site at the 5' end and a polyA signal at the 3' end. Cre recombinase removed the loxP-flanked sequence, leaving behind a loxP site and downstream artificial exon with a splice acceptor, Flag epitope coding sequence, stop codon and c-fos sequence. Western blot analysis confirmed the absence of wild-type protein in testis lysates from homozygous mice. (J:204433)

Generation of the Slirp^tm1.2Pjle allele

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slirp Mutation: 20 strains or lines available

References

• Original: J:204433 Colley SM, et al., Loss of the nuclear receptor corepressor SLIRP compromises male fertility. PLoS One. 2013;8(8):e70700
• All: 1 reference(s)