Ikbkg^tm1.1Dwat
Targeted Allele Detail

Summary

• Symbol: Ikbkg^tm1.1Dwat
• Name: inhibitor of kappaB kinase gamma; targeted mutation 1.1, Derek W Abbott
• MGI ID: MGI:5538732
• Synonyms: X^NemoKi
• Gene: Ikbkg Location: ChrX:73436896-73497460 bp, + strand Genetic Position: ChrX, 38.0 cM
• Alliance: Ikbkg^tm1.1Dwat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:202106
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exons 2 through 5 were replaced with a N-terminally Myc-tagged cDNA containing nucleotide substitutions that result in the amino acid substitution of arginine for lysine at positions 285 and 399 (K285R, K399R). Flp-mediated recombination removed an FRT- and loxP-flanked neomycin resistance cassette inserted downstream of the inserted cDNA. The protein product is nonubiquitinatable and phenotypic analysis indicates a null allele. (J:202106)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ikbkg Mutation: 16 strains or lines available

References

• Original: J:202106 Jun JC, et al., Innate immune-directed NF-kappaB signaling requires site-specific NEMO ubiquitination. Cell Rep. 2013 Jul 25;4(2):352-61
• All: 1 reference(s)