Samhd1^tm1.2Crs
Targeted Allele Detail

Summary

• Symbol: Samhd1^tm1.2Crs
• Name: SAM domain and HD domain, 1; targeted mutation 1.2, Caetano Reis e Sousa
• MGI ID: MGI:5543255
• Synonyms: Samhd1 ko
• Gene: Samhd1 Location: Chr2:156939454-156977016 bp, - strand Genetic Position: Chr2, 78.0 cM, cytoband H2
• Alliance: Samhd1^tm1.2Crs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201909
• Parent Cell Line: PRX-B6N (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 2. Cre-mediated recombination removed exon 2. Flp-mediated recombination removed the neomycin resistance cassette. Western blot analysis confirmed the absence of protein expression in bone marrow-derived dendritic cells and mouse embryonic fibroblasts. (J:201909)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Samhd1 Mutation: 37 strains or lines available

References

• Original: J:201909 Rehwinkel J, et al., SAMHD1-dependent retroviral control and escape in mice. EMBO J. 2013 Sep 11;32(18):2454-62
• All: 5 reference(s)