Kit^{tm1(cre/ERT2)Dsa}
Targeted Allele Detail

Summary

• Symbol: Kit^{tm1(cre/ERT2)Dsa}
• Name: KIT proto-oncogene receptor tyrosine kinase; targeted mutation 1, Dieter Saur
• MGI ID: MGI:5543260
• Synonyms: c-Kit^CreERT2
• Gene: Kit Location: Chr5:75735647-75817382 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Kit^{tm1(cre/ERT2)Dsa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204737
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Kit^{tm1(cre/ERT2)Dsa} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Kit (MGI:96677)

• Mutation details: A targeting construct with an Frt-flanked neo selection cassette 3' of the cre/ERT2 sequence was used for homologous recombination in ES cells. Cre recombinase was inserted into the ATG site in exon 1 of the Kit locus. Germline transmission of the allele was achieved by crossing to C57BL/J mice. (J:204737)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Kit (mouse) Summary of all recombinase alleles driven by Kit. Comparative matrix view of recombinase activities Alliance mouse Kit gene page

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kit Mutation: 182 strains or lines available

Notes

ES cells = 129S6

References

• Original: J:204737 Klein S, et al., Interstitial cells of Cajal integrate excitatory and inhibitory neurotransmission with intestinal slow-wave activity. Nat Commun. 2013;4:1630
• All: 32 reference(s)