Sox17^{tm4.1(icre/ERT2)Heli}
Targeted Allele Detail

Summary

• Symbol: Sox17^{tm4.1(icre/ERT2)Heli}
• Name: SRY (sex determining region Y)-box 17; targeted mutation 4.1, Heiko Lickert
• MGI ID: MGI:5543602
• Synonyms: Sox17^CreERT2, Sox17^{CreERT2deltaneo}
• Gene: Sox17 Location: Chr1:4561154-4567577 bp, - strand Genetic Position: Chr1, 1.65 cM
• Alliance: Sox17^{tm4.1(icre/ERT2)Heli} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204611
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Sox17^{tm4.1(icre/ERT2)Heli} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sox17 (MGI:107543)

• Mutation details: A targeting vector was designed to delete the translational stop codon of Sox17 and to introduce the codon-optimized cre fused to the triple-mutant (G400V, M543A and L544A) human estrogen receptor ligand binding domain (ERT2) sequence preceded by a viral 2A peptide sequence into exon 5. Homologous recombination in ES cells integrated the cre/ERT2 cassette and Frt-flanked neomycin marker so that the targeted Sox17 allele will produce an bicistronic mRNA that will be co-translationally cleaved into equimolar amounts of Sox17 and cre. Chimeras were crossed to a Flp deleter line to remove the neo selection marker. (J:204611)

Generation of Sox17^{tm4.1(icre/ERT2)Heli}.

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sox17 (mouse) Summary of all recombinase alleles driven by Sox17. Comparative matrix view of recombinase activities Alliance mouse Sox17 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sox17 Mutation: 29 strains or lines available

References

• Original: J:204611 Engert S, et al., The Sox17CreERT2 knock-in mouse line displays spatiotemporal activation of Cre recombinase in distinct Sox17 lineage progenitors. Genesis. 2013 Nov;51(11):793-802
• All: 1 reference(s)