Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm
Targeted Allele Detail
|
Symbol: |
Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 2, David H Gutmann |
MGI ID: |
MGI:5543802 |
Synonyms: |
LSL-f-BRAF, LSL-KIAA1549:BRAF |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
|
Alliance: |
Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm page
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:204469
|
Parent Cell Line: |
EDJ22 (ES Cell)
|
Strain of Origin: |
129S6/SvEvTac
|
|
Allele Type: |
|
Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter, Transactivator) |
Mutation: |
|
Insertion
|
|
|
Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm expresses
2 genes
Knock-in expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
BRAF (673) |
|
|
human |
KIAA1549 (57670) |
|
|
|
|
|
Mutation details: The targeting vector introduced into the locus a floxed neomycin resistance/STOP cassette, tetracycline-regulated transcription activator (tTA), insulator, hCMV*1 promoter, intron, fusion of human KIAA1549 (exons 1 to 16) and BRAF (exons 9 to 18) (KIAA:BRAF 16_9), IRES and EGFP. This fusion was found in pilocytic astrocytomas of young children.
(J:204469)
|
|
Generation of the Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm allele |
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
Carrying any Gt(ROSA)26Sor Mutation: |
991 strains or lines available
|
|
Original: |
J:204469 Kaul A, et al., Conditional KIAA1549:BRAF mice reveal brain region- and cell type-specific effects. Genesis. 2013 Oct;51(10):708-16 |
All: |
2 reference(s) |
|