About   Help   FAQ
Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm
Targeted Allele Detail
Summary
Symbol: Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, David H Gutmann
MGI ID: MGI:5543802
Synonyms: LSL-f-BRAF, LSL-KIAA1549:BRAF
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:204469
Parent Cell Line:  EDJ22 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter, Transactivator)
Mutation:    Insertion
 
Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm expresses 2 genes
 
Mutation detailsThe targeting vector introduced into the locus a floxed neomycin resistance/STOP cassette, tetracycline-regulated transcription activator (tTA), insulator, hCMV*1 promoter, intron, fusion of human KIAA1549 (exons 1 to 16) and BRAF (exons 9 to 18) (KIAA:BRAF 16_9), IRES and EGFP. This fusion was found in pilocytic astrocytomas of young children. (J:204469)
Generation of the Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm allele
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  993 strains or lines available
References
Original:  J:204469 Kaul A, et al., Conditional KIAA1549:BRAF mice reveal brain region- and cell type-specific effects. Genesis. 2013 Oct;51(10):708-16
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
11/12/2024
MGI 6.24
The Jackson Laboratory