Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 2, David H Gutmann |
MGI ID: |
MGI:5543802 |
Synonyms: |
LSL-f-BRAF, LSL-KIAA1549:BRAF |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm page
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Germline Transmission: |
Earliest citation of germline transmission:
J:204469
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Parent Cell Line: |
EDJ22 (ES Cell)
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Strain of Origin: |
129S6/SvEvTac
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Allele Type: |
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Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter, Transactivator) |
Mutation: |
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Insertion
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Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm expresses
2 genes
Knock-in expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
BRAF (673) |
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human |
KIAA1549 (57670) |
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Mutation details: The targeting vector introduced into the locus a floxed neomycin resistance/STOP cassette, tetracycline-regulated transcription activator (tTA), insulator, hCMV*1 promoter, intron, fusion of human KIAA1549 (exons 1 to 16) and BRAF (exons 9 to 18) (KIAA:BRAF 16_9), IRES and EGFP. This fusion was found in pilocytic astrocytomas of young children.
(J:204469)
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Generation of the Gt(ROSA)26Sortm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm allele |
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Gt(ROSA)26Sor Mutation: |
993 strains or lines available
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Original: |
J:204469 Kaul A, et al., Conditional KIAA1549:BRAF mice reveal brain region- and cell type-specific effects. Genesis. 2013 Oct;51(10):708-16 |
All: |
2 reference(s) |
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