Topbp1^tm1.1Zqw
Targeted Allele Detail

Summary

• Symbol: Topbp1^tm1.1Zqw
• Name: topoisomerase (DNA) II binding protein 1; targeted mutation 1.1, Zhao-Qi Wang
• MGI ID: MGI:5544423
• Synonyms: TopBP1^ki, TopBP1^W1147R
• Gene: Topbp1 Location: Chr9:103182414-103227627 bp, + strand Genetic Position: Chr9, 55.19 cM
• Alliance: Topbp1^tm1.1Zqw page

Early embryonic developmental defects in Topbp1^tm1.1Zqw/Topbp1^tm1.1Zqw embryos

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:200981
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

Allele Type: Targeted Mutations: Insertion, Single point mutation Mutation details: Tryptophan 1147 in exon 20, the core indispensable aromatic residue in the ATR activating domain was changed to arginine by introducing a T3439C mutation. In addition an FRT flanked neo cassette was inserted in intron 19. Flp mediated recombination removed the neo cassette leaving a single FRT site in intron 19. Immunoblot analysis confirmed similar levels of protein expressing in heterozygous and wild-type MEFs. (J:200981)

Generation of the Topbp1^tm1.1Zqw allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Topbp1 Mutation: 76 strains or lines available

References

• Original: J:200981 Zhou ZW, et al., An Essential Function for the ATR-Activation-Domain (AAD) of TopBP1 in Mouse Development and Cellular Senescence. PLoS Genet. 2013 Aug;9(8):e1003702
• All: 1 reference(s)