Cish^tm1.2Cdon
Targeted Allele Detail

Summary

• Symbol: Cish^tm1.2Cdon
• Name: cytokine inducible SH2-containing protein; targeted mutation 1.2, Chen Dong
• MGI ID: MGI:5544427
• Gene: Cish Location: Chr9:107173858-107179983 bp, + strand Genetic Position: Chr9, 57.99 cM
• Alliance: Cish^tm1.2Cdon page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204829
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT- and loxP-flanked neomycin resistance cassette was inserted upstream of exon 2. An additional loxP site was inserted into exon 3 downstream of the coding sequence. Flp-mediated recombination removed the neomycin resistance cassette. Cre-mediated recombination removed the coding sequence of exon 2 and 3. Western blot analysis confirmed the absence of protein expression on CD4+ T cells. (J:204829)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cish Mutation: 23 strains or lines available

References

• Original: J:204829 Yang XO, et al., The signaling suppressor CIS controls proallergic T cell development and allergic airway inflammation. Nat Immunol. 2013 Jul;14(7):732-40
• All: 2 reference(s)