Spink1^tm3(cre)Kymm
Targeted Allele Detail

Summary

• Symbol: Spink1^tm3(cre)Kymm
• Name: serine peptidase inhibitor, Kazal type 1; targeted mutation 3, Ken-ichi Yamamura
• MGI ID: MGI:5544431
• Synonyms: Spink3^cre
• Gene: Spink1 Location: Chr18:43861134-43870302 bp, - strand Genetic Position: Chr18, 23.47 cM, cytoband B3
• Alliance: Spink1^tm3(cre)Kymm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205182
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Spink1^tm3(cre)Kymm expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Spink1 (MGI:106202)

• Mutation details: A loxJTZ17 site was inserted at the 5' end of a cassette containing the NLS-cre-polyA sequence (nuclear localization signal-cre recombinase-polyadenylation signal). A PGK-puromycin selection cassett and a lox2272 site was inserted at the 3' end of the targeting construct. Targeted recombination in ES cells already containing Spink3^tm1Kymm inserted the cre cassette upstream of exon 1 of Spink3, replacing the neo marker. Chimeras were produced from correctly targeted ES cell clones and germline transmission was established by crosses to C57BL6/J animals. (J:205182)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Spink1 (mouse) Summary of all recombinase alleles driven by Spink1. Comparative matrix view of recombinase activities Alliance mouse Spink1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spink1 Mutation: 14 strains or lines available

References

• Original: J:205182 Sakata K, et al., Generation and analysis of serine protease inhibitor kazal type 3-cre driver mice. Exp Anim. 2014;63(1):45-53
• All: 4 reference(s)