Rab38^em4Rkuhn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rab38^em4Rkuhn
• Name: RAB38, member RAS oncogene family; endonuclease-mediated mutation 4, Ralf Kuhn
• MGI ID: MGI:5544452
• Synonyms: Rab38^IDG-Cht
• Gene: Rab38 Location: Chr7:88079481-88140780 bp, + strand Genetic Position: Chr7, 49.19 cM
• Alliance: Rab38^em4Rkuhn page

Compared with the black fur of a wild-type littermate (pup 159), Rab38^em4Rkuhn/Rab38^em4Rkuhn mice (pup 160) exhibit the dark brown coat color characteristic for chocolate mutants

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Strain of Origin: (FVB/NCrl x C57BL/6NCrl)F1

Mutation description

Allele Type: Endonuclease-mediated Mutation: Nucleotide substitutions Mutation details: TALEN-targeted homologous recombination using a synthetic oligodeoxynucleotide created a nucleotide substitution (G to T) that results in the amino acid substitution of valine for glycine at position 19 (G19V) and thereby created an additional SexAI restriction site. An identifying neutral nucleotide replacement in codon 19 (T to A) was used as a unique sequence identifier. Silent exchanges within the TALEN binding region removed an endogenous ApaLI restriction site. The G19V mutation replicated the amino acid substitution observed in Rab38^cht. This allele was identified in a line established from founder mouse Rc6-4. (J:205086)

Generation of the Rab38^em4Rkuhn allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rab38 Mutation: 15 strains or lines available

References

• Original: J:205086 Wefers B, et al., Direct production of mouse disease models by embryo microinjection of TALENs and oligodeoxynucleotides. Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):3782-7
• All: 1 reference(s)