Hand1^tm2.1Jcc
Targeted Allele Detail

Summary

• Symbol: Hand1^tm2.1Jcc
• Name: heart and neural crest derivatives expressed 1; targeted mutation 2.1, James C Cross
• MGI ID: MGI:5544632
• Synonyms: Hand1^TH
• Gene: Hand1 Location: Chr11:57719539-57722973 bp, - strand Genetic Position: Chr11, 35.27 cM
• Alliance: Hand1^tm2.1Jcc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:202357
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A cDNA containing the tethered homodimer sequence followed by a flexible linker was inserted into exon 1 downstream of the start site methionine. Cre-mediated recombination removed the floxed puromycin resistance cassette inserted downstream of exon 1. Western blot analysis confirmed the absence of monomer protein in E10.5 or E8.5 embryos. (J:202357)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hand1 Mutation: 15 strains or lines available

Notes

This allele was originally generated in ES cells heterozygous for Hand1^tm1Jcc.

References

• Original: J:202357 Hu D, et al., The basic helix-loop-helix transcription factor Hand1 regulates mouse development as a homodimer. Dev Biol. 2013 Oct 15;382(2):470-81
• All: 1 reference(s)