Sirt1^tm1.1Vlcg
Targeted Allele Detail

Summary

• Symbol: Sirt1^tm1.1Vlcg
• Name: sirtuin 1; targeted mutation 1.1, Velocigene
• MGI ID: MGI:5544792
• Gene: Sirt1 Location: Chr10:63154784-63174814 bp, - strand Genetic Position: Chr10, 32.57 cM
• Alliance: Sirt1^tm1.1Vlcg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:200671
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A conditional by inversion (COIN) intron consisting of a lox66_SA-Egpf-polyA_lox71 was inserted in the antisense orientation into exon 3 via homologous recombination, splitting exon 3 into 2 new exons (141 and 101 bp in length). The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the inserted sequence. In addition, an FRT flanked drug selection cassette (hygromycin- or neomycin-delta-thymidine kinase cassette was inserted after the COIN intron. Flp-mediated recombination removed the drug selection cassette. (J:200671)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sirt1 Mutation: 81 strains or lines available

References

• Original: J:200671 Economides AN, et al., Conditionals by inversion provide a universal method for the generation of conditional alleles. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3179-88
• All: 1 reference(s)