Sox10^tm1.1Sout
Targeted Allele Detail

Summary

• Symbol: Sox10^tm1.1Sout
• Name: SRY (sex determining region Y)-box 10; targeted mutation 1.1, E Southard-Smith
• MGI ID: MGI:5544794
• Synonyms: Sox10^tm1.1Vlcg
• Gene: Sox10 Location: Chr15:79039113-79048690 bp, - strand Genetic Position: Chr15, 37.7 cM
• Alliance: Sox10^tm1.1Sout page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:200671
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A conditional by inversion (COIN) intron consisting of a lox66_SA-Egpf-polyA_lox71 was inserted in the antisense orientation into intron 3 via homologous recombination, splitting intron 3 into 2 new exons (94 and 2,258 bp in length). The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the inserted sequence. In addition, an FRT flanked drug selection cassette (hygromycin- or neomycin-delta-thymidine kinase cassette was inserted after the COIN intron. Flp-mediated recombination removed the drug selection cassette. (J:200671)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sox10 Mutation: 33 strains or lines available

References

• Original: J:200671 Economides AN, et al., Conditionals by inversion provide a universal method for the generation of conditional alleles. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3179-88
• All: 1 reference(s)