Rnf7^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rnf7^{tm1c(EUCOMM)Wtsi}
• Name: ring finger protein 7; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5545787
• Synonyms: rbx2 fl
• Gene: Rnf7 Location: Chr9:96352990-96360728 bp, - strand Genetic Position: Chr9, 50.63 cM, cytoband D2.3
• Alliance: Rnf7^{tm1c(EUCOMM)Wtsi} page
• IMPC: Rnf7 gene page

Mutation origin

• Mutant Cell Line: EPD0057_2_C04
• Germline Transmission: Earliest citation of germline transmission: J:205551
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: An FRT-flanked cassette with a splice acceptor, lacZ gene, neomycin resistance gene and polyadenylation sequence (en2SA-LacZ-neo-pA) followed by a 3' loxP site was inserted upstream of the second coding exon. An additional loxP site was inserted downstream of the second coding exon. Flp-mediated recombination removed the lacZ/neomycin resistance cassette and left the second coding exon floxed. (J:205551)

Expression

In Mice Carrying this Mutation:	76 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnf7 Mutation: 17 strains or lines available

Notes

Cell line EPD0057_2_C04 was successfully used to make chimeric mice. Germline transmission was accomplished. J:205551

References

• Original: J:205551 Simo S, et al., Rbx2 regulates neuronal migration through different cullin 5-RING ligase adaptors. Dev Cell. 2013 Nov 25;27(4):399-411
• All: 3 reference(s)