Dll4^tm3.1Vlcg
Targeted Allele Detail

Summary

• Symbol: Dll4^tm3.1Vlcg
• Name: delta like canonical Notch ligand 4; targeted mutation 3.1, Velocigene
• MGI ID: MGI:5545924
• Synonyms: Dll4^i3T2ACOIN
• Gene: Dll4 Location: Chr2:119156286-119166145 bp, + strand Genetic Position: Chr2, 59.97 cM, cytoband E3
• Alliance: Dll4^tm3.1Vlcg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:200671
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: A conditional by inversion (COIN) intron consisting of a lox66_SA-Egpf-polyA_lox71 was inserted in the antisense orientation into intron 3 via homologous recombination, splitting intron 3 into 2 new exons (631 and 144 bp in length). A T2A peptide leads into the marker (TMT2AeGFP). The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the inserted sequence. In addition, an FRT flanked drug selection cassette (hygromycin- or neomycin-delta-thymidine kinase cassette was inserted after the COIN intron. Flp-mediated recombination removed the drug selection cassette. (J:200671)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dll4 Mutation: 27 strains or lines available

Notes

This allele was originally generated in ES cells containing Gt(ROSA)26Sor^{tm3.1(cre/ERT2)Vlcg}.

References

• Original: J:200671 Economides AN, et al., Conditionals by inversion provide a universal method for the generation of conditional alleles. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3179-88
• All: 1 reference(s)